Research Paper Volume 15, Issue 5 pp 1343—1357

Joint effect of THBS2 and VCAN accelerating the poor prognosis of gastric cancer

Long Wang1, *, , Li Feng1, *, , Linnan Liu1, , Jing Han1, , Xue Zhang1, , Dan Li1, , Jiayin Liu1, , Yudong Wang1, , Jing Zuo1, , Zhisong Fan1, ,

  • 1 Department of Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, PR China
* Equal contribution

Received: December 2, 2021       Accepted: February 6, 2023       Published: February 13, 2023
How to Cite

Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Objective: Gastric cancer is the most common malignant tumor of the digestive system. The progression from gastritis to gastric cancer may be related to genetic factors, but the specific molecular mechanism remains unclear. Therefore, an in-depth study of the molecular mechanism of gastritis and gastric cancer is significant.

Methods: We downloaded two gene profiles, GSE2669 and GSE116312, from the Gene Expression Omnibus (GEO) database. This study aims to apply bioinformatics technology to mine differentially expressed genes (DEGs), DEGs annotation, protein-protein interaction (PPI) network creation, and hub gene identification and expression between gastric cancer patients and gastritis patients. Overall survival analysis of hub genes, analysis by comparative toxicogenomics database for hub genes in gastric cancer, THBS2 and VCAN protein expression by immunohistochemistry for gastric cancer and gastritis as well as design of the biological process (BP) neural network was implemented.

Results: The MSLN, SPP1, THBS2, SPARC, FN1, IGFBP7, VCAN were up-regulated in gastric carcinoma samples, while FGA was down-regulated. The protein expression of THBS2 and VCAN in gastric cancer was significantly higher than that in gastritis. VCAN protein expression was positively associated with tumor invasion (P = 0.011) and HER2 overexpression (P = 0.031). Strong correlation among THBS2, VCAN, and gastric cancer based on the BP neural network.

Conclusion: THBS2 and VCAN may be potential targets for improving gastric cancer patients' diagnosis and clinical efficacy.


GEO: Gene Expression Omnibus; DEGs: differentially expressed genes; PPI: protein-protein interaction; BP: biological process; ECM: extracellular matrix; DAVID: Database for Annotation, Visualization and Integrated Discovery; CC: cellular component; MF: molecular function; KEGG: Kyoto Encyclopedia of Genes and Genomes; STRING: Search Tool for the Retrieval of Interacting Genes; MCODE: Molecular Complex Detection tool; CTD: comparative toxicogenomics database; PBS: phosphate-buffered saline; EDTA: Ethylene Diamine Tetraacetic Acid; EBV: Epstein-Barr virus.