Research Paper Volume 16, Issue 3 pp 2077—2089
Lactobacillus plantarum ST-III culture supernatant protects against acute alcohol-induced liver and intestinal injury
- 1 Department of Gastroenterology, Ningbo Medical Center Li Huili Hospital, The Affiliated Hospital of Ningbo University, Ningbo 315000, China
- 2 Healthcare Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
- 3 Department of Infection Diseases, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo 315700, China
- 4 School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
- 5 Ruian People's Hospital, Wenzhou Medical College Affiliated Third Hospital, Wenzhou 325200, China
- 6 School of Mental Health, Wenzhou Medical University, Wenzhou 325035, China
- 7 Department of Pharmacy, Ningbo Medical Center Li Huili Hospital, The Affiliated Hospital of Ningbo University, Ningbo 315000, China
- 8 School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China
Received: July 18, 2023 Accepted: November 6, 2023 Published: December 19, 2023
https://doi.org/10.18632/aging.205331How to Cite
Copyright: © 2024 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The beneficial effects of probiotics have been studied in inflammatory bowel disease, nonalcoholic steatohepatitis, and alcoholic liver disease (ALD). Probiotic supplements are safer and more effective; however, their potential mechanisms are unclear. An objective of the current study was to examine the effects of extracellular products of Lactobacillus plantarum on acute alcoholic liver injury. Mice on a standard chow diet were supplemented with Lactobacillus plantarum ST-III culture supernatant (LP-cs) for two weeks and administered alcohol at 6 g/kg body weight by gavage. Alcohol-induced liver injury was assessed by measuring plasma alanine aminotransferase activity levels and triglyceride content determined liver steatosis. Intestinal damage and tight junctions were assessed using histochemical staining. LP-cs significantly inhibited alcohol-induced fat accumulation, inflammation, and apoptosis by inhibiting oxidative stress and endoplasmic reticulum stress. LP-cs significantly inhibited alcohol-induced intestinal injury and endotoxemia. These findings suggest that LP-cs alleviates acute alcohol-induced liver damage by inhibiting oxidative stress and endoplasmic reticulum stress via one mechanism and suppressing alcohol-induced increased intestinal permeability and endotoxemia via another mechanism. LP-cs supplements are a novel strategy for ALD prevention and treatment.