Research Paper Volume 2, Issue 2 pp 93—100

Figure 2. DNA from aged subjects is demethylated compared to DNA from young subjects. (A) FlashGel showing the molecular weight of Aged and young DNA. Figure is representative of eight such experiments. (B) Bar diagram depicting the damage in DNA from aged and young subjects as determined by ELISA that measures the number of abasic sites per 10⁵ base pairs. Figure is mean + S.E. of twenty five separate aged and young DNA tested. (C) Bar diagram depicts the percent of global methylation in aged and young DNA as measured by ELISA. The methylated fraction of DNA is recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. Figure is mean + S.E. of twenty four separate aged and young DNA tested.