Research Paper Volume 2, Issue 10 pp 691—708
Regulatory roles of tankyrase 1 at telomeres and in DNA repair: suppression of T-SCE and stabilization of DNA-PKcs
Figure 5. Time course of tankyrase 1 (TNKS1), tankyrase 2 (TNKS2) and DNA-PKcs relative mRNA expression following tankyrase 1 siRNA depletion. Quantitative RT-PCR of mRNA at 4, 8, 12, 18, 24 and 48 hr demonstrates dramatic reduction of tankyrase 1 mRNA (confirming efficiency of knockdown), as well as no significant reduction of tankyrase 2 (confirming specificity of knockdown) or DNA-PKcs (all p < 0.05).