Research Paper Volume 2, Issue 11 pp 815—822
Core transcription factors, Oct4, Sox2 and Nanog, individually form complexes with nucleophosmin (Npm1) to control embryonic stem (ES) cell fate determination
Figure 2. Sox2 physically interacts with Npm1 in ES cells. (A) Immunofluorescence confocal microscopy in combination with in situ PLA showed that there is an interaction between Sox2 and Npm1 in ES cells. Complexes (red dots) were detected in the nucleoplasm of interphase cells. DNA was counterstained by Hoechst 33342 (blue). Scale bar represents 10 μm. (B) Co-immunoprecipitation experiments followed by Western blot analysis showed that Npm1 can be immunoprecipitated using anti-Sox2 (1 M NaCl and 0.1 M Citrate).