Research Paper Volume 3, Issue 1 pp 33—43
HIF-1α antagonizes p53-mediated apoptosis by triggering HIPK2 degradation
Figure 1. HIF-1α antagonizes p53 apoptotic activity. (A) Luciferase assay in C38 and C27 prostate cancer cells showed significantly higher HIF-1α-luc promoter activity in C27 cells. *P=0.0159. (B) Luciferase assay of p53AIP1-luc reporter in C38 and C27 prostate cancer cells after bleomycin (Bleo) treatment showing impairment of p53 transcriptional activity in C27 cells. (C) Immunoblot of C38 and C27 cells after 40 Gy X-irradiation revealed PARP cleavage and Ser46 phosphorylation only in C38 cells. Blot image was cut and pasted. (D) RT-PCR analysis of p53 apoptotic target genes in C38 and C27 prostate cancer cells after bleomycin (Bleo) treatment. Gel image was cut and pasted. (E) Luciferase assay of p53 target Noxa-luc reporter in C38 cells after X-irradiation revealed impairment of luciferase activity after HIF-1α overexpression. (F) Immunoblot of RKO cells after adriamycin (ADR) treatment showed abolishment of (p)Ser46 after HIF-1α-Flag overexpression. (G) Luciferase assay of p53AIP1-luc reporter in C27 cells showed induction of luciferase activity after HIF-1α silencing with siRNA.