Figure 1. Acute and chronic effects of resveratrol (RSV) on glucose-stimulated insulin secretion in INS-1E β-cells. Acute effects of RSV (A). Following a 2h pre-incubation period without glucose, INS-1E cells were stimulated for 30 min in KRBH with 2.5 or 15 mM glucose (Glc) in the absence (Control) or presence of 1, 5, and 25 μM of RSV. Values are means ± SE of 6 independent experiments. *p<0.05, **p<0.01 versus 2.5 mM Glc of the corresponding group; §p<0.05 versus Control group at 15 mM Glc. Chronic effect of sulfonylureas and RSV (B). INS-1E cells were cultured for 24h in the absence (Ctl) or the presence of 1 μM glibenclamide (Glib), 250 μM tolbutamide (Tolb), 5 μM DIDS, and 25 μM RSV. Next, cells were washed and pre-incubated without drugs and without glucose for 2h. Then, cells were incubated for 30 min in the absence of the tested compounds at 2.5 or 15 mM Glc. Values are means ± SE of 3 independent experiments. * p<0.05, **p<0.01 versus 2.5 mM Glc of the corresponding group; §p<0.05 versus Ctl group at 15 mM Glc.