Research Paper Volume 3, Issue 10 pp 985—1002

Figure 2. Age-dependent expression levels of SIRT1 in blood and tissue samples of C57/B6 mice. Western blot analysis of SIRT1 expression in age groups from 2 days (d) to 25 months (m); panels (A) SIRT1 expression in plasma samples normalized with selected Ponceau S stained band, showing constant levels for all samples used, along with histograms presenting average densitometric values, (B) SIRT1 expression in PBMC samples normalized with β-actin, along with histograms presenting average densitometric values, (C) SIRT1 expression in brain samples normalized with β-actin, along with histograms presenting average densitometric values, (D) Composite graph presenting SIRT1 expression in plasma, PBMC and brain samples. (*p < 0.01, **p < 0.0001; all histograms represent Mean ± SD; n = 3; three different mice used from each selected age group.)