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Research Paper Volume 3, Issue 10 pp 955—967

Nuclear protein accumulation in cellular senescence and organismal aging revealed with a novel single-cell resolution fluorescence microscopy assay

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Figure 3. Co-staining of total protein and histone H3. Cell we stained with NanoOrange (NO) and subsequently processed for immunofluorescent detection of histone H3 (H3) as indicated in Methods. (A) Representative images acquired with cultures of HDF. The panels are arranged and labeled as indicated in Figure 2A. (B) Representative images acquired with cultures of MTF. The panels are arranged and labeled as indicated in (A) above.