Figure 1. Defining mTOR-dependent CS. (A) Measurement of CS. 80000 HT-p21-9 cells were plated in 0.2 ml medium per well in 96-well plate with 500 nM rapamycin (R) or without rapamycin – control (C). After 4 days, the plate was photographed to record color of media (i.e. pH). The color of medium without cells is shown for comparison n/c (no cells). Then cells from each well were split into larger wells of 6-well plates.