Figure 2. Cytotoxicity of conditioned medium (CM) and lactate. HT-p21-9 cells (A) and HCT116 cells (B) were plated in 96-well plates at low density (2000 cells/well) and treated with a range of lactic acid (LA) concentrations or conditioned medium (CM), collected from overgrown HT-p21-9 cell culture. After 3 days, medium was changed to fresh medium and cells were allowed to proliferate for 5 days and then number of viable cells was determined using MTT assay. CM, R-CM, and CM+R were prepared as described in Methods.