Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 2. SPARC expression in young and old rat SMPCs. (A) qPCR analysis of SPARC expression in SMPCs from young and old rats 3 days after plating. Data are expressed as the mean ± standard error of the mean (SEM) (n = 4, respectively). (B) Immunoblotting analysis of SPARC expression levels in SMPCs from young and old rats 3 days after plating. Data are expressed as mean ± SEM (n = 3, respectively). (C) Representative immunostaining experiments to detect SPARC expression in SMPCs from young and old rats 3 days after plating (left). Scale bar = 100 μm. A plot of the proportions of nuclei in SPARC-positive cells is shown (right). Data are expressed as the mean ± SEM (n = 3, respectively).