Research Paper Volume 4, Issue 1 pp 40—48

Figure 5. Effects of siSPARC on the myogenic potential of SMPCs. (A) Representative immunostaining of myogenin (above) and quantitative analysis of myogenin-positive cells (below). Nuclei were counterstained with Hoechst 33258. White arrowheads indicate myogenin-positive nuclei. Scale bar = 100 μm. Proportions of myogenin-positive cells were calculated and ratios to. control values are graphed. Data are expressed as mean ± SEM (n = 4, respectively). *p < 0.05 vs. control. (B) Representative immunostaining of MHC (above) and quantitative analysis of MHC-positive cells (below). Nuclei were counterstained with Hoechst 33258. White arrowheads indicate MHC-positive cells. Scale bar = 100 μm. Proportions of nuclei in MHC-positive cells were calculated and ratios to control values are graphed. Data are expressed as mean ± SEM (n = 4, respectively). *p < 0.05 vs. control.