Research Paper Volume 4, Issue 3 pp 206—223

Figure 2. dSir2 regulates fat metabolism. (A) Total body triglyceride (TAG) in dSir2 mutants (Sir2^2A.7.11) and whole body dSir2^RNAi (+ RU486) flies, with respective controls (n = 36). (B) Oil-Red O staining of fatbodies from control and Sir2^2A.7.11. (C) Relative expression of fat metabolism genes lipase-3 (lip3), brummer (brmm), mitochondrial acyl carrier protein (mtACP), medium chain acyl CoA dehydrogenase (mcad), aceto acetyl CoA thiolase (ACoT), long chain acyl CoA dehydrogenase (lcad), acetyl CoA carboxylase (ACC), fatty acid synthase (fas) and diacyl glycerol synthase (dDAG) (n = 24). 200 μM RU486 was used to knockdown dSir2 expression in pSw-tub-gal4>dSir2^RNAi flies. Student's t-test was used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).