Research Paper Volume 4, Issue 5 pp 368—374

Figure 3. Integrin α5 knock-down and inhibition of EGFR exert different yet overlapping effects on cell cycle distribution of A431 cells. Cells treated with PD168393 as detailed in Fig. 2 (A) or transduced with control or α5 shRNA-containing vectors (B) were seeded for 48-72 h, then fixed and prepared for flow cytometry as indicated in “Materials and methods”. FACS analysis was performed on a FACS-Calibur (Bekton Dickinson). Shown are typical experiments.