Research Paper Volume 4, Issue 9 pp 636—647
Down regulation of miR-124 in both Werner syndrome DNA helicase mutant mice and mutant Caenorhabditis elegans wrn-1 reveals the importance of this microRNA in accelerated aging
Figure 6. Impact of vitamin C on ROS, ATP, and lipofuscin levels in wild type and mutant C. elegans strains. (A) ROS levels in whole body of 7-day adult old worms (post-larval L4 stage) detected with dichlorofluorescein. Data are expressed as units of fluorescence per milligrams of proteins. (Unpaired Student's t-test; *P < 0.00005; ** P = 0.00009). (B) ATP levels in whole body of 7-day old adult worms (post-larval L4 stage). (Unpaired Student's t-test; *P = 0.00972; **P = 0.0002). Twenty-five worms of each genotype were collected for the ROS or ATP measurements. Experiments were performed in triplicate. (C) Histogram representing the average intensity of autofluorescence lipofuscin in the different C. elegans strains treated with vitamin C compared to untreated wild type worms. Ten to fifteen three-days old (three days into adulthood) worms of each strain were photographed and the fluorescence intensity was quantified using Adobe Photoshop.