Research Paper Volume 5, Issue 1 pp 37—50
Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues
Figure 7. Sudan Black B (SBB) staining demonstrates lipofuscin accumulation in lung adenomas (AD) and absence in adenocarcinomas (AdCa), in formalin-fixed paraffin-embedded (FFPE) lung sections from mice conditionally expressing K-rasV12. (A) Haematoxylin and Eosin staining demonstrates a lung adeoma (AD) and an adenocarcinoma (AdCa) on the same FFPE section (B) Histological features of the adenoma and the adenocarcinoma shown in A section. (C) Characteristic perinuclear deposition of blue black granules in adenoma cells stained with Sudan Black B (SBB), representing positivity for lipofuscin, while adenocarcinoma cells are negative for SBB. (D) Fluorescence microscopy (at 450-490 nm) verifying lipofuscin presence in the adenoma, and absence in the adenocarcinoma. Scale bars: A, 600 μm; B-D, 100 μm. Inset: Cells at higher magnification.