Research Paper Volume 4, Issue 12 pp 899—916

Figure 4. Levels of pS6 and p-AKT in the hearts of 10 months old mice: control, fasted, rapamycin. (A) Immunoblot analysis of protein lysates from the hearts of females and males. Numbers above blots are individual mice in each group. Standard loading (SL) – 10 microgram lysates from cultured cells were loaded onto each upper and lower gels and blots were exposed to comparable intensities in SL lanes,. Fasted: mice were fasted for 16 h and sacrificed, control: mice were fed ad libitum, Rapa: “control” mice were treated with 1.5 mg/kg rapamycin (i.p.) 1 h before sacrifice. (B, D) – Quantified intensities of pS6 and p-Akt signal in hearts of females versus males. Female and male groups were comprised of all animals un-treated with rapamycin (n = 9 in female group and n = 11 in male group) regardless of feeding status. Data are mean ± SE. (C, E) Quantified intensities of pS6 and p-Akt signal in each sub-group. Mean ± SE in each subgroup separately: F – fasted; C – control; R – rapamycin-treated subgroup.