Research Paper Volume 5, Issue 3 pp 151—154
Sirt1 activation by resveratrol is substrate sequence-selective
Figure 1. Activation of Sirt1 by resveratrol is substrate sequence-selective. (A) Sirt1-dependent deacetylation of microarray peptides in presence of resveratrol, relative to their deacetylation without compound. Shown is the negative logarithm of the ratio of residual acetylation signals. (B) Effect of resveratrol on Sirt1-dependent deacetylation of peptides from three substrate classes – deacetylation stimulated, unaffected, or inhibited – tested in solution using mass spectrometry. (C) Dose-dependent activating effect of resveratrol on the deacetylation of a SF38-K23 peptide by Sirt1. (D) Sequence features of peptides whose deacetylation by Sirt1 is either activated or inhibited through resveratrol. The acetyllysine is in the center, residues on top are favored, residues at the bottom disfavored when compared to their overall frequency at this position.