Research Paper Volume 5, Issue 9 pp 643—652

Figure 3. Increased numbers of myeloid progenitor cells in aged CD137^−/− and CD137L^−/− mice. Bone marrow cells from 3 and 12 months old WT, CD137^−/− and CD137L^−/− mice were stained for lineage marker, IL-7R, CD117 and Sca-1. Cells were first gated on the progenitor cell region based on forward and side scatter. Then lineage marker⁻, IL-7R⁻ cells were gated out for analysis of myeloid progenitors (CD117⁺ Sca-1⁻) and KSL (CD117⁺ Sca-1⁺) cells. (A) Gating strategy of KSL cells and myeloid progenitor cells. (B,C) Number of (B) KSL cells and (C) myeloid progenitor cells in 3 and 12 months old mice. Each group consisted of at least 3 mice. Data are representative of two independent experiments. *p<0.05.