Research Paper Volume 6, Issue 4 pp 264—280
Prelamin A accumulation and stress conditions induce impaired Oct-1 activity and autophagy in prematurely aged human mesenchymal stem cell
Figure 5. Prelamin A accumulation and serum starvation conditions induce the overexpression of Oct-1 and its impaired activity in hMSCs. (A) Representative confocal immunofluorescence staining showing the expression of Oct-1 and prelamin A in hMSCs under basal (unstarved) or serum starvation conditions (starved). Scale bar: 20 μm. (B) Luciferase reporter assays of Oct-1 transcription factor activity in hMSCs cultured under under basal (unstarved) or serum starvation conditions (starved). Bars are average +/- standard deviation of 3 independent donors. Differences that are significant are marked as follows: ** p<0.01 when compared to control-hMSCs, && p<0.01 when compared to control-hMSCs starved.