Research Paper Volume 6, Issue 8 pp 645—660

Figure 5. Effects of ubiquitous knock down of Sin3A on expression of genes known to impact longevity and glutathione synthesis. RT-qPCR performed using cDNA prepared from RNA isolated from whole flies from control (-RU486), SIN3 KD or GFP RNAi (+RU486). Fold changes in gene expression for GFP RNAi, SIN3 KD I and II are shown for predicted longevity relevant genes in seven day- (A) or 20 day-old flies (B) and genes important for the de novo synthesis of glutathione in seven day-old flies (C). Error bars represent standard error of the mean. * p<0.05.