Research Paper Volume 6, Issue 8 pp 661—674
Role of mitochondrial reactive oxygen species in age-related inflammatory activation of endothelium
Figure 3. Antioxidants (0.2 nM SkQ1, 1 mM NAC, 200 μM Trolox) prevent TNF-induced activation of EA.hy926 endothelial cells. (A, B) Expression of ICAM1 mRNA in TNF-induced (4 h, 50 pg/ml) endothelial cells. (C) Cell-surface ICAM1 expression and IL-6 and IL-8 cytokine secretion in TNF-induced (8 h, 5 ng/ml) endothelial cells (n = 3). (D) Neutrophil adhesion to endothelium monolayer stimulated with TNF (8 h, 5 ng/ml). c, control. Data are represented as mean +/− SEM; n = 3 except for 0.2 nM SkQ1 data on Figs. 3A-3C, where n ≥ 15. * p ≤ 0.05, ** p < 0.001,*** p < 0.0001.