Figure 9. The expression of p21/waf1 mRNA and protein in WT and GMF-TG mice. (A) The expression of p21/waf1 mRNA increased in the kidneys of the GMF-TG mice at 10 weeks, compared with the WT mice. The data is shown as means ± S.E. (10w WT; n=3, 10w GMF-TG; n=4). *; P < 0.05 vs. 10w WT mice. (B) In the kidneys at 60 weeks, the expression of p21/waf1 mRNA in the GMF-TG mice was reduced significantly, compared to that of the WT mice at the same age. The data is shown as means ± S.E. (60w WT; n=4, 60w GMF-TG; n=4). **; P < 0.01 vs. 60 w WT mice. (C) This figure shows the results from western blot analyses of the p21/waf1 protein in the kidney of the WT and GMF-TG mice at 10 and 60 weeks. The data shown is representative data on the estimated ratios of p21/waf1 to α-actin, in the case of equivalent protein loading within a gel. Increased p21/waf1 protein levels were detected in the GMF-TG mice at 10 weeks, compared with the WT mice. However, there was no difference in the levels of p21/waf1 protein between the GMF-TG and WT mice at 60 weeks. The data is shown as means ± S.E. (10, 60w WT; n=3, 10, 60w GMF-TG; n=3). *; P < 0.05 vs. 10w WT mice. N.S.; not significant versus 60w WT mice.