Figure 5. NSun2-p27 regulatory process impacts on the progression of replicative senescence. (A) 2BS cells were stably infected with a lentivirus bearing a pHBLV-NSun2 vector (pNSun2) or a pHBLV-shNSun2 vector (shNSun2). Cell lysates were prepared and subjected to Western blot analysis to assess the levels of proteins NSun2, p53, CDC25C, p27, p16, CDK1, and GAPDH. (B) RNA prepared from cells described in Fig.