Research Paper Volume 8, Issue 1 pp 34—47

Sirt6 regulates dendritic cell differentiation, maturation, and function

Figure 1. Sirt6 regulates the generation of cDCs in vivo and in vitro. (A, B) WT or Sirt6KO BM cells were cultured with GM-CSF and their ability to generate CD11c+ cells was analyzed at day 3, 5, 7 by flow cytometry. (A) The percentage of CD11c+ cells within cultures of Sirt6KO BMs (day 7) was normalized to that of WT CD11c+ cells. Results are presented are means ± SEM of 6 separate experiments, n=13 for each genotype; ***: p<0.001. (B) one representative experiment out of two is presented. (C-E) BM cells from WT and Sirt6KO mice were analyzed by flow cytometry for the frequency of cDC precursors (pre-cDCs, CD11c+MHCII), pDCs, monocyte/macrophage subsets, mature granulocytes and of BM progenitors of different lineages. (C) one representative experiment out of six is presented; (D, E) results are presented are means ± SEM of fifteen and six separate experiments, respectively, n=6-15 for each genotype; *: p<0.05; **: p<0.01; n.s.: not significant. (F) TNF-α concentration in the supernatants of WT and Sirt6KO BMDCs (harvested at day 6) were determined by ELISA. Results are means ± SEM of three separate experiments, n=10 for each genotype; *: p<0.05. (G) WT and Sirt6KO BM cells were cultured with GM-SCF with or without addition of the indicated concentrations of TNF-α. CD11c+ cells were quantified at day 6 by flow cytometry. One representative experiment out of six is presented, n=6-9 for each genotype.