Research Paper Volume 8, Issue 1 pp 95—110

Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes

Figure 1. Longevity is inversely associated with testicular COX2 expression and PGD2 production. Panel (A) COX2 (72 kDa) expression was evaluated by immunoblotting in testicular homogenates of short-lived (GH-Tg) and long-lived (GHRH-KO and Ames dwarf) mice. Bar plot graphs represent the mean ± S.E.M. and depict the quantification by densitometry of the bands. Results were normalized to actin (42 kDa) and expressed as fold change relative to the control (normal littermates), which was assigned a value of 1. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test. Panel (B) PGD2 testicular levels were determined by immunoassay in testicular homogenates from short-lived (GH-Tg) and long-lived (GHRH-KO and Ames dwarf) mice. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test.