Research Paper Volume 8, Issue 1 pp 95—110

Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes

Figure 4. Decreased longevity is associated with increased testicular lipid peroxidation and antioxidant enzymes expression. Panel (A) testicular lipid peroxidation was determined using the Thiobarbituric Acid Reactive Substances (TBARS) assay. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test. Panel (B) testicular mRNA expression of superoxide dismutase 1 (SOD1), catalase (CAT), peroxiredoxin1 (PXR1) and glutathione peroxidase (GPX) was determined by real time-PCR. Results were normalized to GAPDH housekeeping gene and expressed as fold change relative to the control (normal littermates), which was assigned a value of 1. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test. Panel (C) Testicular protein expression of catalase (60 kDa) was measured by immunoblotting. Bar plot graphs represent the mean ± S.E.M. and depict the quantification by densitometry of the bands. Results were normalized to actin (42 kDa) and expressed as fold change relative to the control (normal littermates), which was assigned a value of 1 (n = 5-7). * p < 0.05, t-Student test.