Research Paper Volume 8, Issue 1 pp 158—176

To senesce or not to senesce: how primary human fibroblasts decide their cell fate after DNA damage

Figure 2. Dynamics of p16, Rb, Cyclin A2 and Cdc25A expression after γ-irradiation in MRC5 human fibroblasts. (A) Relative p16 abundance. (B) Relative Rb1(Ser780) abundance (C) Total and hypo-phosphorylated Rb1 protein for 2.5 Gy IR. (D) Total and hypo-phosphorylated Rb1 protein for 10 Gy IR. (E) Relative Cyclin A2 abundance (F) Relative Cdc25A abundance. Error bars indicate SEM (n≥3). Representative Western Blots are shown in Figure S6, Supplemental Figures.