Research Paper Volume 8, Issue 2 pp 245—259
Hexokinase is a key regulator of energy metabolism and ROS activity in insect lifespan extension
Figure 5. Developmental expression and ChIP assay. (A) and (B) Har-CREB and -POU expression patterns during pupal stages. Brain proteins (20 μg) were extracted and used for Western blotting analysis. DP, diapause-type pupae; NP, nondiapause-type pupae. (C) Har-POU, -CREB and -c-Myc ChIP assays testing Har-HK promoter binding. In, input; IP, DNA immunoprecipitated using anti-Har-POU, -CREB and -c-Myc antibodies; Ec, empty control immunoprecipitated using pre-immune serum; Mock, mock control immunoprecipitated using anti-Har-actin antibody.