Research Paper Volume 8, Issue 2 pp 272—290

Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease

Figure 2. The rs11014002 SNP promotes miR-603 biogenesis. (A) Predicted secondary structure model and ΔG analysis of miR-603 precursor (pre-miR-603) wild type, pre-miR-603 rs11014002 and pre-miR-603 rs79500031. (B) Quantitative RT-PCR analysis of miR-603 expression in the precursor group (left) and immunoblot analysis of GFP expression to confirm transfection efficiency (right). (C) Quantitative RT-PCR analysis of miR-603 expression in the primary transcript group (left) and immunoblot analysis of GFP expression to confirm transfection efficiency (middle and right). ***P < 0.001 versus the wild type, ###P < 0.001 versus the rs11014002 as determined by One-way ANOVA with a Bonferroni's multiple comparison test. *P < 0.05 versus the vector as determined by paired two-tailed Student's t-tests (B right).