Figure 2. Senescent cells upregulate MICA and ULBP2 on the cell surface. (A) ImageStream analysis demonstrates higher expression levels of MICA and ULBP2 on the cell surface membrane of DIS IMR-90 fibroblasts (Senescent) compared to control (Growing) cells. (B) Quantification of total intensity and total membrane intensity indicate higher levels of MICA and ULBP2 in DIS IMR-90 cells compared to growing (control) cells. (C) Representative immunofluorescence staining of MICA and ULBP2 performed on non-permeabilized cells demonstrates higher expression levels of these proteins on the cell surface membrane in DIS WI38 and BJ cells compared to growing (control) cells. Scale bar: 50μm. MICA and ULBP2 are shown in red. DAPI is shown in blue. Data presented as mean with S.E.M of three independent experiments. Two-tailed t-test *P<0.05, **P<0.001, ***P<0.0001.