Research Paper Volume 8, Issue 3 pp 484—505

Figure 8. Mir-15b regulates mitochondrial ROS generation and the mitochondrial membrane potential in a SIRT4-dependent manner in primary human dermal fibroblasts. Mitochondrial ROS levels (A) and the mitochondrial membrane potential (B) were determined by staining with cell-permeable dyes and flow cytometric analysis as described in Materials & Methods and by Kalfalah et al. [102]. Shown are mean ± s.d. values from four to six experiments. (C) Mitochondrial ROS levels were determined in the presence of the mitochondria-specific antioxidant mitoQ [72]. Cells were analyzed four days following transfection of miR-15b inhibitors in the absence or presence of siRNA duplexes against SIRT4. Depicted are mean ± s.d. values from four to six experiments. To evaluate statistical significance, ANOVA on ranks Dunn´s tests were performed (*p<0.05; ***p<0.001).