Research Paper Volume 8, Issue 4 pp 685—694

Figure 3. SIRT1, 2, 3 are essential for spindle morphology in aged oocytes. (Aa) Spindle morphology in fresh MII oocytes. (Ab; Ac) Spindle morphology in aged oocytes. DNA (red); Spindle (green). Scale bars: 10 μm. (B) Percentages of abnormalities in control or NAM-treated groups at 12h and 24h after aging, respectively. (C) The proportion of abnormal spindles in control or caffeine-treated oocytes at 12h and 24h of aging, respectively. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05).