Research Paper Volume 8, Issue 5 pp 889—898

Figure 2. Trends in autofluorescence over time in individual C. elegans. (a) Computationally straightened brightfield and fluorescence images of two different individuals (left and right) at selected time-points are shown. The timecourse for each individual is shown top-to-bottom, with images annotate by the number of hours before/after that individual's death. Fluorescence images obtained in the “red”, “green”, and “blue” channels (see Figure 1) are shown individually and in false-color merge images for each time-point. Note the peak in green and blue fluorescence near each individual's time of death (as ascertained by cessation of movement following stimulation). (b) Whole-body fluorescence across the entire experiment is shown for the two individuals pictured in panel a. For each fluorescent channel, the mean and 95^th percentile intensity of all image pixels in the animal's body is plotted over time.