Research Paper Volume 8, Issue 4 pp 796—809

Naturally occurring mitochondrial-derived peptides are age-dependent regulators of apoptosis, insulin sensitivity, and inflammatory markers

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Figure 6. Circulating SHLP2 levels decrease with age in mice and protect against amyloid-beta (Aβ) toxicity. (A) Plasma SHLP2 levels in young and old male and female C57BL/6 mice (n = 10 in each group) were assessed by ELISA. (B) Mouse primary cortical neurons were treated with vehicle control, 10 μM Aβ1–42 alone, or co-treated 10 μM Aβ1–42 with 1 nM, 100 nM, or 10 μM SHLP2. Aβ1–42-induced cytotoxicity was measured using a LDH (lactate dehydrogenase) cytotoxicity assay kit.