Research Paper Volume 8, Issue 7 pp 1384—1397

Figure 5. FOXM1 regulates oxidative stress in epithelial squamous cell carcinoma. (A) A253 cells were silenced for p63, and relative mRNA (48 h after transfection) and protein (96 h after transfection) levels of FOXM1 and p63 were determined. Values reported are the average ± SD of two independent experiments. (B) Time-course analysis of ROS levels in A253 cells silenced for FOXM1 for 96 h and treated with 200 μM hydrogen peroxide. The ROS level was measured by FACS. Values reported are the average of two independent experiments. *p-value <0.05 by Student's t-test. Western blot confirming the silencing. (C) Cells were silenced for FOXM1 for 96 h and treated with 200 μM hydrogen peroxide. The ROS level was measured by FACS at 24 h after treatment. Values reported are the average ± SD of three independent experiments. *p-value <0.05 and **p-value <0.001 by Student's t-test. (D) Cells were silenced for FOXM1 for 96 h and treated with 2 μM doxorubicin. The percentage of sub-G1 events was measured by FACS at 24 h after treatment. Values reported are the average ± SD of three independent experiments. *p-value <0.05 by Student's t-test.