Research Paper Volume 8, Issue 8 pp 1650—1669
Ionizing radiation-mediated premature senescence and paracrine interactions with cancer cells enhance the expression of syndecan 1 in human breast stromal fibroblasts: the role of TGF-β
Figure 4. Invasive human breast cancer cells MDA-MB-231 enhance SDC1 expression in stromal fibroblasts in a paracrine mode. Early-passage (young: Y) and irradiation-mediated senescent (IS) human breast stromal fibroblasts were incubated with media conditioned by the MDA-MB-231 and MCF-7 human breast cancer cell lines (CM MDA-MB-231 and CM MCF-7, respectively). Twenty four hours later SDC1 expression was assessed by real-time PCR. One representative experiment out of three similar ones is depicted (* indicates p < 0.05 in comparison to untreated cells).