Research Paper Volume 8, Issue 10 pp 2308—2323

Figure 5. SIRT6 regulates p27 via its deacetylation activity. (A) Schematic representation of full-length SIRT6 and its central core domain deleted mutant ΔSIRT6. (B) In vivo ubiquitination assay of p27 influenced by SIRT6 deleted mutant ΔSIRT6. (C) Overexpress SIRT6 and its mutants R65A (lack deacetylase activity) and G60A (lack mono-ADP-ribosyltransferase activity) in 293 cells, then protein levels of p27 was detected. (D) In vivo acetylation assay of p27 was carried out after overexpressing and knocking down SIRT6 in 293 cells. (E) The acetylation level of p27 was detected after overexpressing wild-type SIRT6 and its deletion ΔSIRT6.