Research Paper Volume 9, Issue 1 pp 256—285

Melatonin-micronutrients Osteopenia Treatment Study (MOTS): a translational study assessing melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7) on bone density, bone marker turnover and health related quality of life in postmenopausal osteopenic women following a one-year double-blind RCT and on osteoblast-osteoclast co-cultures

Figure 10A-F. Effect of MSDK on RUNX2, β1 integrin, NFκB and metabolic proteins. After 21 days of MSDK treatment, western blot was performed to determine (A) RUNX2 expression of osteoblasts grown in transwell co-cultures, (B) RUNX2 expression of osteoblasts grown in layered co-culture, (C) β1 integrin expression of osteoblasts grown in transwell co-cultures, (D) β1 integrin expression of osteoblasts and osteoclasts grown in layered co-cultures (E) NFκB expression of osteoclasts grown in transwell co-cultures, and (F) NFκB expression of osteoblasts and osteoclasts grown in layered co-cultures.