Research Paper Volume 9, Issue 3 pp 778—789

Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

Figure 8. (A) Western blot showing that BMP2, annexin A6 and annexin A2 carriage to be higher in MVs from senescent than from young HUVEC. Equal protein loading was confirmed using a HSP90 antibody. Total protein expression was obtained via densitometric analysis and expressed as the ratio protein/HSP90 in arbitrary units. n=3 pools; *p<0.05; **p<0.01. (B) Representative BMP2, annexin A6 and annexin A2 western blot of young and senescent HUVEC pools. Equal protein loading was confirmed probing with GAPDH or β-Actin. The graphs present densitometric band analysis normalized to GAPDH or β-Actin in arbitrary units. n=3 pools.