Research Paper Volume 9, Issue 3 pp 914—931

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer

Figure 1. Rab1B and MMP9 are overexpressed in CRC cell lines. (A) The expression of Rab1B and MMP9 proteins in a panel of CRC cell lines and an immortalized colon cell line is determined by immunoblot. GAPDH is used as a loading control. (B) Relative expression of Rab1B and MMP9 mRNA (normalized to GAPDH) in the same set of cell lines as in (A) is examined by real-time quantitative PCR. (C) The correlation between Rab1B and MMP9 proteins (normalized to GAPDH) in the CRC cell lines was determined by Spearman correlation assay. (D) Spearman correlation analysis is used to analyze the correlation between Rab1B and MMP9 mRNAs in CRC cell lines.