Figure 2. Western blot for SMAD4, FRAT1 and BCL2 protein expression after deregulation of miR-34a-3p in Ben-Men-1 cells. (A, B, C) Representative Western blot images for SMAD4, FRAT1 and BCL2 protein expression 48 h after transfection of Ben-Men-1 cells with control (scrambled), miR-34a-3p (mimic) or anti-miR-34a-3p (inhibitor) using β-actin as loading control. (D, E, F) Quantification of SMAD4, FRAT1 and BCL2 protein expression relative to β-actin protein expression determined by Western blot. Values are means ± SD of three independent experiments (*, P < 0.05; **, P < 0.01).