Research Paper Volume 9, Issue 3 pp 986—998

Mouse mitochondrial lipid composition is defined by age in brain and muscle

Figure 4. Brain mitochondrial fatty acid composition decreases with ageing. (A) Characterisation of fatty acid species that decrease in the ageing brain mitochondria. 94% of the fatty acids identified (Supplementary Table 5) decrease with ageing in the brain mitochondria. 48% of the fatty acids found to decrease were identified as polyunsaturated fatty acids (PUFA), 24% were saturated fatty acids (SFA), 24% monounsaturated fatty acids (MUFA) and a small percentage, 4%, were identified as hydroxy fatty acids (HFA). (C) Representative scatter plot for the lipid m/z 357.2796, tentatively identified as PUFA C22:5. This lipid shows a significant decrease in abundance in 78 week old brain mitochondria (n=10) compared to 4-11 week old brain mitochondria (n=10), p=0.048. (D) Representative scatter ploy for the lipid m/z 393.3732, tentatively identified as a C26.1 MUFA shows a decrease in abundance aged brain mitochondria. (E) Representative scatter plot for the saturated fatty acids. The lipid m/z 339.3264, tentatively identified as SFA C22:0 decreases in abundance in aged brain mitochondria. (F) Representative scatter plot for the lipid m/z 295.2274 tentatively identified as HFA C18 H31 O3. This lipid decreases in abundance in aged brain mitochondria. Scatter plots display abundance ± SEM. We used Wilcoxon Rank test and Bonferroni correction. Refer to Supplementary Table 5.