Figure 7. LOC100129148's oncogenic activity is in part through negative regulation of miR-539-5p, and then activation of KLF12 in NPC cells. (A) The 3'-UTR of KLF12 harbors one miR-539-5p cognate site. (B) Relative luciferase activity of reporter plasmids carrying wild-type or mutant KLF12 3'-UTR in CNE-1 and SUNE-1 cells co-transfected with negative control (NC) or miR-539-5p mimic. (C) mRNA expression of KLF12 and miR-539-5p in NPC tissues. (D) KLF12 is highly expressed in NPC tissues (T) that NP tissues (N). (E) Relative miR-539-5p expression after transfection with miR-NC and miR-539-5p. (F) Relative KLF12 mRNA expression after transfection with miR-NC and miR-539-5p. (G) Relative KLF12 protein expression after transfection with miR-NC and miR-539-5p. Assays were performed in triplicate. *P < 0.05. Means ± SEM are shown. Statistical analysis was conducted using student One-Way ANOVA test.