Research Paper Volume 9, Issue 4 pp 1153—1185

The age- and sex-specific decline of the 20s proteasome and the Nrf2/CncC signal transduction pathway in adaption and resistance to oxidative stress in Drosophila melanogaster

Figure 12. Hydrogen peroxide stress resistance improves with continual knockdown of Keap1 in aged (60 days) flies. In panels A and B, male and female progeny of the Actin-GS-255B strain crossed to Keap1 RNAi strain were collected and aged to 60 days. (A) Females raised in the absence of RU486 were either pretreated with 10µM H2O2 (gray line) “10µM H2O2” or not pre-treated with H2O2 (black line) “No Additions”. Females raised in the presence of RU486 were either pretreated with 10µM H2O2 (red line) “10µM H2O2 (+RU486)” or not pretreated with H2O2 (pink line) “+RU486”. (B) Males raised in the absence of RU486 were either pretreated with 10µM H2O2 (gray line) “10µM H2O2” or not pre-treated with H2O2 (black line) “No Additions”. Males raised in the presence of RU486 were either pretreated with 10µM H2O2 (blue line) “10µM H2O2 (+RU486)” or not pretreated with H2O2 (green line) “+RU486”. Statistical difference in survival (p < 0.05) was calculated using the Log-Rank test. Statistical summary is located in Supplementary Table S4.