Research Paper Volume 9, Issue 4 pp 1153—1185

The age- and sex-specific decline of the 20s proteasome and the Nrf2/CncC signal transduction pathway in adaption and resistance to oxidative stress in Drosophila melanogaster

Figure 5. The adaptive expression of the 20S proteasome is age and tissue-dependent in females. Body segments collected from females of the Actin-GS-255B strain crossed to the w[1118] strain were used as controls, or were pretreated with either 10µM or 100µM hydrogen peroxide. (A,B) 20Sα expression in female abdominal tissue following pretreatment. (A) 3 day old. (B) 60 day old. (C,D) 20Sα expression in female head following pretreatment. (C) 3 day old. (D) 60 day old. (E,F) 20Sα expression in female thorax following pretreatment. (E) 3 day old. (F) 60 day old. Western blots were performed in triplicate, normalized to Actin-HRP, and quantified using ImageJ. The bar charts represent the quantification. Error bars denote standard error of the mean (S.E.M) values. * P <0.05, ** P <0.01, *** P < 0.001, relative to the female control using one-way ANOVA. Statistical significance is indicated in young females with pink asterisks (pink *).