Research Paper Volume 9, Issue 4 pp 1153—1185

The age- and sex-specific decline of the 20s proteasome and the Nrf2/CncC signal transduction pathway in adaption and resistance to oxidative stress in Drosophila melanogaster

Figure 7. Males show no tissue-specific or age-related adaptive changes in 20S proteasome protein levels. Body segments were collected from males of the Actin-GS-255B strain crossed to the w[1118] strain that were used as controls, or that were pretreated with either 10µM or 100µM hydrogen peroxide. (A,B) 20Sα expression in male abdominal tissue following pretreatment. (A) 3 day old. (B) 60 day old. (C,D) 20Sα expression in male head following pretreatment. (C) 3 day old. (D) 60 day old. (E,F) 20Sα expression in male thorax following pretreatment. (E) 3 day old. (F) 60 day old. Western blots were performed in triplicate, normalized to Actin-HRP, and quantified using ImageJ. The bar charts represent the quantification. Error bars denote standard error of the mean (S.E.M) values, relative to the male control using one-way ANOVA.