Research Paper Volume 9, Issue 4 pp 1186—1201

Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes

Figure 4. Effects of supplementation with U0126, ceramide or BDNF during in vitro aging on STAS of mouse oocytes. Cumulus-denuded oocytes (DOs) recovered 13 h post hCG injection were used. For U0126 and BDNF treatment, the DOs were aged for 36 h in CZB medium supplemented with different concentrations of U0126 or BDNF before ethanol and 6-DMAP treatment for activation. For ceramide treatment, DOs were microinjected with 6 pl DMSO alone (control) or DMSO containing 1 mM ceramide and then aged for 36 h in CZB medium before ethanol and 6-DMAP treatment for activation. Each treatment was repeated 4-5 times with each replicate containing about 30 oocytes. a,b: Values with a different letter above bars differ significantly (P < 0.05).