Research Paper Volume 9, Issue 4 pp 1326—1340

BMP2 promotes proliferation and invasion of nasopharyngeal carcinoma cells via mTORC1 pathway

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Figure 4. BMP2 activates mTOR signal pathway in NPC cells. (A) Western blotting analysis revealed that in S18-BMP2 and 6-10B-BMP2 cells, the expression of phosphorylated (active) mTOR, P70S6K and 4EBP1 (p-mTOR, p-p70S6k and p-4EBP1) were upregulated compared to those control cells. (B) The expression of p-mTOR, p-p70S6k and p-4EBP1 were downregulated in 5-8F knockdown BMP2 cell. (C) Proliferation abilities of S-18 and 6-10B cells expressing BMP2 or the empty vector was evaluated by CCK8 assay after pretreatment with or without 20 nM of rapamycin. (D) Invasive abilities of S-18 and 6-10B cells expressing BMP2 or the empty vector was evaluated by transwell assay after pretreatment with or without 20 nM of rapamycin.