Research Paper Volume 9, Issue 4 pp 1326—1340

BMP2 promotes proliferation and invasion of nasopharyngeal carcinoma cells via mTORC1 pathway

Figure 4. BMP2 activates mTOR signal pathway in NPC cells. (A) Western blotting analysis revealed that in S18-BMP2 and 6-10B-BMP2 cells, the expression of phosphorylated (active) mTOR, P70S6K and 4EBP1 (p-mTOR, p-p70S6k and p-4EBP1) were upregulated compared to those control cells. (B) The expression of p-mTOR, p-p70S6k and p-4EBP1 were downregulated in 5-8F knockdown BMP2 cell. (C) Proliferation abilities of S-18 and 6-10B cells expressing BMP2 or the empty vector was evaluated by CCK8 assay after pretreatment with or without 20 nM of rapamycin. (D) Invasive abilities of S-18 and 6-10B cells expressing BMP2 or the empty vector was evaluated by transwell assay after pretreatment with or without 20 nM of rapamycin.